Here are the topics covered in the most recent issues of Staying Current: Formulation of Biopharmaceuticals: Please select the option below, if you'd like to subscribe. April 2010 Fundamentals of Sterile Packaging Systems High Throughput Formulation Screening for MABs Use of DLS to Measure Viscosity Viscosity of Concentrated Mab Solutions Rational Mab Formulation Development Thiols and Disulfides I Protein Stability Importance of the Mobile Phase in SEC Analysis SEC and Arg in the Mobile Phase PTH(1-34) and Microneedle Patches Influenza Vaccine on Microneedle Patches Quantitation of Trehalose Crystallinity Protein Aggregation using Microcalorimetry ITC in Bioseparations and Fermentation Protonation and Fibrillation Met Oxidation of PTH (1-34) Dry Powder Formulation of bFGF Thermal Stability of ∝Lactalbumin Polymerization of Neuroserpin Aggregation-Resistant ßSheet Folding Heat Transfer at the Protein-Water Interface Urea Denaturation of Proteins A ßAggregation Kinetics Shear Precipitation of a Mab Protein Stability in Ionic Liquids Capillary Electrophoresis of IL-7 Silicone Oil Emulsions Aggregation of ∝1-Antitrypsin Signal Peptide Heterogeneity in Mabs Nonisothermal Stability of Insulin Nonisothermal Stability of Cholecystokinin Peptide Flow-Induced Structural Changes Liquid Formulations of Antivenoms May 2010 Stabilization of Dried Vaccine Vectors Gatekeeper Residues and Aggregation Predictive Tool for Amyloid Sequences Fluorescent Molecular Rotor Dyes Fluorescence Studies of Fibrillation Protein Stability Thermal Unfolding of Enzymes Glycosylation of Therapeutic Proteins Electrophoresis of PEGylated Proteins PEGylated Endostatin PEG Characterization by GEMMA and MS Salts and Colloidal Stability Self-Interaction Chromatography High Concentration Lyo Formulations Trehalose-Glycerol Matrices Collapse and Protein Stability Sugar Alcohols as Glass Formers Peptide Mapping of IgG1 Antibodies Chemical Instability in Hybrid Antibodies Mass Spec and Antibody Deamidation L-Proline Stabilization of IVIG Highly Glycosylated Interferon-∝ Mass Spec and Conformational Stability Pre-Screening G-CSF Formulations Lys Modification and Fibrillation Thermal Aggregation of Glycated BSA Shortcomings of SEC Analysis Identifying the Amyloid-like Fibril Trehalose and BSA Stability Novel Enzyme Stabilizers Formulation Design Space Aggregates of Dehydrogenases Met Oxidation and Aggregation June 2010 Crystallization of Lysozyme SIC of a Monoclonal Antibody Protein-Protein Interactions in Ovalbumin Colloidal Stability and Hofmeister Series Evaluating Protein Stability Lysozyme-Salt Interactions Protein Aggregation in Salt Solutions Salt-Induced Gelation of Proteins SEC and IgG1 Aggregation Inhibiting IgG Aggregation by Peptic Fragments Detecting IgG Aggregation by Extrinsic Fluorescence Stability of IgG Subtypes CZE of Monoclonal Antibodies Stabilization with Substituted Cyclodextrins ⋎-Irradiation and Terminal Sterilization Polyols as Stabilizers of PAH Oxidation of Free L-Histidine Citrate Binding to a Fab Fragment Mobility in Oligosaccharides Assay(s) for Polysorbate 80 Lyophilization of Bacteriophage Excipients for Pulmonary Delivery Protein Aggregation Pathways Populations of Amyloid Protofibrils ∝-Synuclein Aggregation Protein Interfacial Hydration Stability of Protein Crystals Ultrafiltration of a Self-Associating Protein
July 2010 Quantitation of Protein Aggregation Stirring-Induced Particulates of MAbs Shaking Stress in Preformulation of Mabs CEX and Antibody Physical Stability Stability of a Plantibody in Tobacco Leaves SEC Method for Interferon Alpha 2 Preparative IEF for Antibodies Improved PAGE Method SDS-PAGE of VH Domains Albumin Pharmaceutical Preparations Denatured State and Conformational Stability Analysis of Polysorbate 80 Dewetting and Unfolding of Proteins Aggregation Nucleus Size Structure in Urea-Denatured Ensembles Prediction of Aggregation Prone Regions Kinetics of Antibody Chemical Degradation Fragmentation of an Antibody Protein Unfolding of ß-Lactoglobulin Using Raman Trehalose Crystallization Stabilization of a Lyophilized Enzyme Trends in Lyophilization Nanoparticle Tracking Analysis Subunit Vaccine for Inhalation Insulin Unfolding by 2-D IR Disulfide Susceptibility
August 2010 Micro-Flow Imaging (MFI) of Proteins MFI of Opalescent Antibody Solutions MFI of a High Concentration Mab Aggregates in Repackaged Bevacizumab Subvisible Particles in Protein Products Prediction of Amyloidogenic Sequences Aggregation-Prone Regions in CDRs Liquid-Liquid Phase Separation of a IgG1 Analysis of Thermal Unfolding Profiles Lag Time and Aggregation Kinetics Enzyme Destabilization by HP-ß-CD Adsorption during Sterile Filtration High-Throughput Formulation of Mabs VCD of Proteins Improved Solubility of Mabs Colloidal Stability of Lysozyme Monitoring Biomanufacturing with Raman Radical-Induced Hinge Cleavage in Mabs Aggregation of Cystatins Flexibility in Antibodies Stability Engineering of scFvs
September 2010 Sequence Comparison of a Biosimilar MAb. Glycosylation of a Biosimilar tPA Effect of pH on Thermal Stability of MAbs IR to Monitor Protein Structure H-D Exchange and IR Spectroscopy Chain Scission at Asp Residues Mass Spec of Asp and isoAsp Products Ficoll 70 as an Enzyme Stabilizer Crowding and Cytochrome c Stability Aggregation during Protein Purification Aggregation and Membrane Fouling ß-Lactoglobulin Aggregation Fibrillar Polymorphism (2) ß-Lactoglobulin Fibril Formation Aggregation of SOD Mutants Benzyl Alcohol-Induced Aggregation Protein Kinetic Stability Zinc Binding and Endostatin Stability ATP-Induced Unfolding of Lysozyme Microfluidic Rheology of Proteins Protein Refolding Using Microfluidic Chips Improved Reproducibility of Fibrillation Behavior of Spray-Dried Insulin IR Spectra of Proteins in KBr Water Clusters and Protein Stabilization Protein Phase Behavior Flexibility and Prion Stability Aggregates in IVIG Solutions ∝-Crystallin as an Excipient Protein Stability in Ammonium Carbamate CEX of IgG1 Charge Variants
