Here are the topics covered in the most recent issues of Staying Current: Formulation of Biopharmaceuticals: Please select the option below, if you'd like to subscribe.
January 2011 Amyloidogenesis of IgG Light Chains Met Oxidation of MAbs Effect of Oxidation on Protein Structure Hemoglobin Structure in Electric Fields Deamidation and Electric Fields Leachable from a Protein in I.V. Bags Glycation of Immunoglobulin G Methylglyoxal Reaction with ∝-Crystallin CD Spectra of Peptides in Films Mass Spectrometry of Amyloid Interfacial Packing of Proteins on Surfaces Shear Forces and Unfolding of vWF Dental Formulations of bFGF Deamidation and Solubility of Hordein Trp Residue and Staph. Nuclease Stability Rigidity and Thermostability Amyloidogenic Intermediates DNA Formulation Development Prediction of Disulfides Conformation of Disulfides Patterns around Disulfides Hydration and Acid Unfolding Thermostabilization of an Esterase Thermal Behavior of ß-Lactamase Specificity of Fibril Formation Light Chain Amyloidosis Aß(1-42) Fibril Stability IAPP Fibril Formation Instability and Bioprocessing Cofactor Stabilization of bR Ethanol-Induced Aggregation IDP Fusion Proteins Aggregation of Myosin Subfragments Cys Sulfenic Acid and Disulfide Bonds GnHCl-Induced Aggregation Acid-Induced Aggregation of MAbs
February 2011 Subvisible Particles in an IgG2 Preparation Adsorption of MAbs to Glass Studying MAb Aggregation with AFM H-D Exchange Studies of Fibril Formation Nucleation and Fibril Polymorphism Removing Insulin Microaggregates Enzyme in Polyurethane Foam Oxidation and Conformational Stability (2) pKa Values in the Unfolded State Mobility in the Glassy State Solid State Stability and Glycosylation Primary Drying Rates and Protein Stability Coated Microneedles and Protein Stability Influenza Vaccine and Microneedles Vaccines and Aluminum Salt Adjuvants HTS of Aluminum-Adjuvanted Antigens Immunogenicity of Protein Therapeutics Ligand-Induced Changes in Unfolding Degradation of Polysorbates High Pressure Unfolding of Proteins Urea and Dry Globule States of Proteins Phase Separation in Lyophilisates Nebulization of Dornase AlfA Crowding and Protein-Protein Binding Interactions of Crowding Agents
March 2011 Carrier Systems for Polypeptides Injectable Thermosetting Hydrogel Conjugation of Peptides to Macromolecules Thermoresponsive Gels & Protein Delivery Nanoporous Microparticles from Sugars Surface Tension and Protein Stability Polyols and Stability of G-CSF Aggregation of Amelogenin Protein Aggregation Premolten Globule State in Cytochrome c New Sugar-Based Surfactants Ion Exchange Chromatography of MAbs Stability of ∝-Amylases upon Glycation Effect of Salts on Protein Viscosity SDS-Induced Denaturation of Lipase Surfactants and ∝-Lactalbumin Oxidation of Dairy Proteins MS Methods for Monitoring Oxidation RNase A Oligomer Formation Neutron Scattering of Sugar-Lysozyme ß-CDs in Lyophilized Formulations Sucrose-Polymer Lyo Formulations Aggregation of a Bot Protein Antigen Protein Adsorption to Biopolymers Stability of Allergens during Processing High Hydrostatic Pressure Refolding Field-Flow Fractionation Phase Transitions in Folded Proteins Fibroin Hydrogels of MAb Delivery Proteins in Silica Matrices
April 2011 Correction (Hoehne et al.) Shear Effects on Protein Stability m-Cresol-Induced Aggregation siRNA Delivery to the Lung Flow Cytometry and Silicone Oil Flow Cytometry and Subvisible Particles bis-ANS and Thermally Stressed IgG1 High Throughput Solubility Screening Ficin Denaturation High-throughput Screening of Formulations Selective Accumulation of Ions on Proteins Alternative IgG4-Fc Structures Comparability Studies of IgG1 MAbs Dansyl-PEGs as Peptide Stabilizers Asp-Asp Isomerization and Racemization Disaccharides and a Lyophilized Enzyme Freeze-Thaw Stability of MCP-1 Enzymatic Fragmentation of a MAb Removal of MAb Aggregates using CHT Stability of a Proline-Stabilized IgG Product Aggregation and Complement Activation Thermostability and Salt Bridge Formation Prediction pf Protein Binding Regions GlycoPEGylation of Factor VIIa Protein Stabilization by Zinc Ion Disulfides and Fibril Formation Aggregation of ⋎D Crystallin Crowding Effects on Protein Structure Crowding Effects on Amyloid Formation 12 Destabilization of Protein by Osmolytes Monitoring Aggregation with NMR Aggregation Pathways in IAPP Aggregation of ApoA-I Mutants Analysis of Aß Fibrils by CE Properties of Lyophilized Cakes by NIR Detecting Phase Separation by Raman Moisture-Induced Aggregation of TT
May 2011 Assessing Protein Aggregates Modeling Amyloid Formation Aggregation of ⋎S-Crystallin Chemical Modification and Stabilization Osmolytes and Denaturants Effect of Osmolytes on a Model Peptide Saccharides and Protein Stability Hydration and Peptide Stabilization Immunogenicity Assessment Frozen Storage of a Monoclonal Antibody Primary Drying Effects on Proteins Effect of Metal Ions on Aß Fibrillation Two Distinct Aggregation Pathways for Aß Repeated Seeding of Aß Fibrillation Aß Intermediates by NMR Whey Protein-Surfactant Interactions Thermal Aggregation of Wine Proteins UF/DF of Monoclonal Antibodies Nanoparticle Detection of Aggregation Pharmacopeial Standards PEGylation of Insulin Ice-Induced Damage: Membrane Protein Interactions and Phase Separation NIR as a PAT Tool June 2011 Degradation of Polysorbates Aggregation of IgG1 at low pH (2) High Throughput Screening of MAbs Protein-Protein Interactions in MAbs Fluorescence Single Particle Tracking Protein-Surfactant Interactions Current Uses of Trehalose Effect of Syringe Surfaces on Proteins Immunogenicity and Aggregation Insulin Fibrillation and Teflon Litrate Adducts of Oxytocin Oxytocin Stabilization by Metals and Citrate Injectable Microspheres of IFN-?2b Respirable Nanoparticles of DNase I Glucagon-Loaded Nanospheres Respirable Protein Powder by Spray Drying PTH(1-34) Powders for Inhalation Resveratrol Inhibits Oligomer Formation Polyoxometalates Inhibit Fibrillation Aggregation of PEGylated Insulin Site-Specific Glycation of a MAb Antibody Analysis of HSA Glycation F-T Stability by H/D Exchange Comparability using H/D Exchange Thermal Inactivation of Lipase Thermal Stability of Tyrosinase Hydrophobic Interactions and Stability Dynamics of Lysozyme-Water Mixtures Solvent of Protein Dynamics Aggregation of BSA Reversible Dissociation of Fibrils Non-Arrhenius Model of Aggregation Container Selection for Peptide Work Transdermal Delivery of Proteins July 2011 Platform Formulation of Proteins Cosolutes and Protein Stability Polyols Stabilization of Proteins Rational Vaccine Formulation Development Small Angle Scattering and Aggregation WAXS and Insulin Denaturation CE-MS of Proteins Salting-In of Proteins Arginine and Protein Aggregation Aromatic Side Chains and Aß Fibrillation Premolten and Molten Globules of Cyt-c Nanoparticle Inhibition of Aß Fibrillation New Analytical Methods for Proteins Thermal Stress Testing of Etanercept Tuning Up Proteins Physical Stability of Oxidized Calcitonin Racemization in the Hinge Region of IgG1 Freezing Step in Lyophilization Effect of Freezing on Buffers Analysis of Mannitol with Raman and NIR Lyophilization of DNA Polyplexes Hydrophobic Hydration and Denaturation Engineering Protein Stability Comparing SEC and AF4 Fibril Polymorphism Denaturation of ∝-Crystallin Ions and the Air/Water Interface Revisiting Ramachandran Plots
August 2011 Delivery of Angiogenic Growth Factors Lyophilization of PEG-hGH Lyophilization Cycle Optimization HIP and Protein Encapsulation into PLGA Microemulsions and Transdermal Delivery Delivery of Peptide-Based Vaccines AF4 Analysis of Proteins and Particles IR Detection of Met Oxidation Conformational Flexibility and Fibrillation Aggregation of ß-Lactoglobulin Stabilization of HRP Stabilization of a MAb with Basic a.a.’s Oxidation of Trp in CDR of a IgG1 PEGylation and BA-Induced Aggregation Effect of CH2 Conformational Stability Insulin Aggregation during Agitation Fragmentation of IgG1 MAbs Nanoparticle Delivery of siRNA O-Glycosylation and Stability of IFN-∝2b Oral Delivery of Insulin Protein Adsorption and Drug Delivery HIC Analysis of an Oxidized IgG1 CE Analysis of Disulfide Scrambling Fibrillation of Myoglobin Mutants Prediction of Aggregation Propensity Dry Heat Induced Aggregation of WPI Intranasal Immunization GAGs Promote Light Chain Fibrillation QbD and Biopharmaceutical Development Unfolding/Aggregation of Lysozyme High Pressure and Protein Flexibility Metal Ions and Aggregation of HSA Formulation of a Live Vaccine Polyol Stabilization of Proteins Polyol Hydrophobicity and a.a. Solubility
September 2011 High Throughput Screening for Stabilizers Classification of Protein Aggregates Chemical Modifications of Aggregates Viscosity of High Concentration BSA Effect of Sugars on the Viscosity of MAbs MAb Aggregation at low pH MAb Aggregation during Manufacturing MAb Compatibility with Pre-filled Syringes High Fidelity Peptide Mapping Glycosylation Status by Raman Surfactant Interactions and Aggregation Aggregates of WPI and ?-Lactoglobulin Monitoring Protein Unfolding by CE Stability of Repackaged MAbs Setting Guidelines for Biosimilars Biosimilar EPOs in Thailand Stabilization of Endoglucanse by EDTA Flexibility and Protein Stability Dry Powder Aerosols for Gene Delivery Moisture-Induced Aggregation Detection of Amyloidogenic Proteins Aggregation of Monoclonal Antibodies Oxidation and Thermal Aggregation Arg and Lysozyme Aggregation Non-Fibrillar Aggregation of ?-Synuclein Heat-Induced Aggregation by MS Thermal Stability Shift Analysis
October 2011 Deamidation Leads to Fibril Formation Monitoring Glycation of IgG1 Chain Scission at Ser Residues Shear Flow Effects on Proteins Coulter Counting and Subvisible Particles Nanoparticle Size Measurements Taylor Dispersion Analysis for Sizing Chiral Amyloid Superstructures Suppression of Aggregation by Arg Post-Production Aggregation of Proteins Quantitation of Aggregation by SV-AUC Predicting MAb Aggregation Rates Thermal Precipitation Assay Denaturation of Transketolase Comparability Strategies for MAbs Subvisible Particles during Filling Effect of Alcohols on Hemoglobin Structure Fibrillation of Insulin Analogs Secondary Structure from Amide VI Bands Stability-Indicating Assay for GM-CSF Characterization of Radiolabeled MAbs 2-D Gels and MALDI-TOF of MAbs Acetontrile-Induced Aggregation of IgGs Radical-Induced Degradation of MAbs Aggregation Detection with a Sensor Minimizing Protein Surface Adsorption
November 2011 Lessons on Protein Stability Stability of Lyophilized ß-Lactoglobulin Sugar Crystallization in Lyophilisates Drying of Invertase in Alginate Beads Development of PEGylated Proteins Stability of Small PEGylated Proteins Measuring B22 Values using DLS Opalescence Behavior of an IgG2 Comparability Assessment of Two MAbs CD Spectroscopy of Proteins IR Spectroscopy of Proteins Salt Effects on Structure by IR Spectroscopy Multidimensional Formulation Effects of Interfaces and Leachables Glass Particles as Adjuvants Aggregation Hotspots near CDRs Calcium Binding to FIX-Fc Fusion Protein Effect of Ca on Polyol Stabilization Degradation of PTH(1-34) Novel Degradation of a PEGylated Protein Stabilization of Calcitonin by Pluronic F68 Benzyl Alcohol and IL-1ra Saccharides and Tg Values Worldwide Biosimilar Development Salt Bridges and Thermostability
